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KEY RESOURCES TABLE

Journal: Neuron

Article Title: Vasomotion as a driving force for paravascular clearance in the awake mouse brain

doi: 10.1016/j.neuron.2019.10.033

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Monoclonal Mouse Anti-Human Smooth Muscle Actin Agilent Cat#M-0851 Goat anti-mouse Alexa Fluor 488 Thermo Fisher Scientific Cat#A-11001 Bacterial and Virus Strains AAV1.Syn.GCaMP6s.WPRE.SV40 UPenn Vector core N/A Chemicals, Peptides, and Recombinant Proteins Methoxy-X04 Glixx Laboratories Inc. CAS#863918-78-9 70 kDa fluorescein Dextran Invitrogen Cat#D-1823 70 kDa Texas Red Dextran Invitrogen CatD-1830 DyLight 594 labeled Lycopersicon Esculentum (Tomato) Lectin Vector Laboratories Cat#DL-1177 CdSSe/ZnS core/shell QDs with PEG Ocean Nanotech LLC Cat#QMG-450–02 Fluorescein sodium salt Sigma-Aldrich Cat#F6377 Experimental Models: Organisms/Strains C57BL/6J mice Jackson Laboratories Stock#000664 B6C3-Tg(APPswe,PSEN1dE9)85Dbo/Mmjax mice Jackson Laboratories Stock#34829-JAX B6.Cg-Tg(Myh11-cre,-EGFP)2Mik/J mice Jackson Laboratories Stock#007742 Software and Algorithms MATLAB Mathworks R2014b-R2016a FIJI Schindelin et al. Nat Methods 2012 ImageJ 1.52e Graphpad Prism www.graphpad.com Version 5.03 SPSS IBM Version 22 Open in a separate window KEY RESOURCES TABLE Spontaneous low frequency oscillations can be observed in arterioles in awake mice Vasomotion drives paravascular clearance of solutes from the brain Paravascular clearance is impaired in the context of cerebral amyloid angiopathy

Techniques: Virus, Plasmid Preparation, Recombinant, Labeling, Software